Functional Assays
& Assay Development
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Functional Assays
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Angiogenesis Assays |
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| Functional
Assays & Assay Development |
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| Angiogenesis Assays |
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| cell proliferation | ||||||||||||||||||||||||||
| cell migration | ||||||||||||||||||||||||||
| cell differentiation | ||||||||||||||||||||||||||
| vascular
sprouting assay |
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| matrigel/FGF assays |
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| tumor models | ||||||||||||||||||||||||||
| Angiogenesis, the formation of new blood
vessels, is thought to play an important role in the establishment and
propagation of primary as well as secondary metastatic tumors (Weidner,
N., et al. N. Eng. J. of Med. 324:1-8. 1991) Several synthetic and naturally occurring inhibitors of angiogenesis have been described. Experimental synthetic inhibitors of angiogenesis such as minocycline, a-difluoromethylornithine, and analogs of heparin and fumagilin have shown promising results in vitro and in some in vivo studies. Naturally occurring inhibitors of angiogenesis such as thrombospondin and platelet factor 4, both isolated from blood platelets as well as tissue inhibitor of metallproteinases (TIMP), purified from mesenchymal cells derived from cartilage, have demonstrated potent anti-angiogenic activity as well as anti-proliferative activity for vascular endothelial cells (Passaniti, A., et. al. Lab. Invest. 67:519-528. 1992) More recently, naturally occurring, putative angiogenesis inhibitors including Angiostatin and Endostatin have been described (O'Reilly, M.S., et. al. Cell 79:315-328. 1994 and O'Reilly, M.S., et. al. Cell 88:277-285. 1997). |
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Paragon Bioservices, Inc -
Johns Hopkins Bayview Research Campus Alpha Center - 5210 Eastern Avenue - Baltimore, Maryland 21224 800.545.6569 - 410.550.2919 - 410.550.2924 (fax) NIH BPA#: 263-00054123 - www.paragonbioservices.com - pbi@paragonbioservices.com |
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© 2003 Paragon Bioservices, Inc. |
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To
support companies and academic laboratories in the development of
angiogenic or inhibitory compounds, Paragon has developed an integrated
series of in vitro and in vivo assays