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Functional Assays
& Assay Development
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Angiogenesis Assays
Cell Proliferation
BAEC, HUVEC, HSMC or other
 
96 well format - Catalog #10100 MTT
  • 104 cells/well in basal medium o/n
  • Proliferation induced with bFGF + compound (5 concentrations: 10, 1, 0.1, 0.01, 0.001 mM plus positive and negative controls)
  • Triplicates
  • Cultures maintained for 3 days
  • Data derived from Spectrophotometry - MTT assay
 
24 well format - Catalog #10102 Cell Counts
  • 5 x 104 cells / well in basal medium o/n
  • Proliferation induced with bFGF + compound (5 concentrations: 10, 1, 0.1, 0.01, 0.001 mM plus positive and negative controls)
  • Triplicates
  • Cultures maintained for 3 days
  • Data derived from actual cell counts
 
24 well format - Catalog #10100-B BrdU
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Functional Assays &
Assay Development
 
Angiogenesis
Assays
 
cell proliferation
 
cell migration
 
cell differentiation
 
vascular sprouting
assay
 
matrigel/FGF
assays
 
tumor models
  • 5 x 104 cells / well in basal medium o/n
  • Proliferation induced with bFGF + compound (5 concentrations: 10, 1, 0.1, 0.01, 0.001 mM plus positive and negative controls)
  • Triplicates
  • Cultures maintained for 3 days
  • Data derived from BrdU incorporation
 
Contact Paragon Bioservices to discuss your specific needs
 

Cell Culture & Fermentation Services
Recombinant Protein Expression | Protein Purification
Proteomics Services - Protein Characterization
Virus Production & Purification | Process Development
Functional Assays & Assay Development | Histology & Immunohistochemistry
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800.545.6569 - 410.550.2919 - 410.550.2924 (fax)
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