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Table
of Cell Culture and Fermentation Services
In support of laboratories conducting gene regulation studies, Paragon
Bioservices offers:
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- Hela Cell Pellets
- Hela Nuclear Extracts
- Hela Cytoplasmic Extracts
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| Nuclear Extracts |
| Proteins that bind to DNA, such as transcription factors, are identified using gel shift and footprinting assays and utilizing crude soluble nuclear extracts. The gel shift assay involves electrophoretic retardation of a short DNA fragment in a gel when a protein or a protein complex is bound to it. This simple assay can be used to track the purification and identification of DNA binding proteins from nuclear extracts. The footprinting assay identifies specific DNA sequences to which proteins bind by demonstrating the binding protein's ability to protect a specific region of DNA from modifications or cleavage. In addition to DNA binding proteins, nuclear extracts can be used for
in vitro transcription and RNA splicing assays to study proteins involved in transcription and RNA processing events. |
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| Cytoplasmic Extracts (S-100 Extracts) |
| Identification of some transcription factor complexes also require protein components that are found in the cytoplasmic fraction of cellular preps (ex. RNA polymerase III transcription factors). In addition, S100 extracts are useful for studying nucleic acid binding proteins such as viral or RNA binding proteins. S100 fraction and nuclear extracts can be prepared simultaneously. |
Other cytosolic fractions (S-10, S-15 extracts) as well as purified
Organelle fractions are also available. For more information, see
Purification of Organelles. |
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Paragon
Home Page |
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Cell
Culture &
Fermentation Services |
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Hela
Nuclear Extracts & S100 Preps |
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Monoclonal
Antibody Production |
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Scale-up
Monoclonal Antibody Production & Purification |
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Related
Links |
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Recombinant
Protein Expression |
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Adenovirus
&
AAV Production |
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Purification
of Organelles |
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