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Bacteria & Yeast
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Starting from a gene sequence or cDNA,
Paragon scientists will clone your gene (± affinity tags) into a
commercially available expression vector or a custom vector that you
provide.
Contact Paragon Bioservices to
discuss your project |
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The veracity of constructs will be documented by restriction digest and/or DNA sequencing. Following transformation of expression cell lines (E. coli, S. cervisiae, P. Pastoris) and antibiotic selection of recombinants, the best protein expressing clones will be identified by SDS-PAGE and Western immunoblot. The best clone will be further optimized for protein expression. |
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| Recombinant Protein Expression |
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| Bacteria & Yeast Expression | ||||||||
| Baculovirus
- Insect Cell |
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| Mammalian | ||||||||
| Transfection Services | ||||||||
| Plasmid
DNA Production & Purification |
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Paragon Bioservices, Inc -
Johns Hopkins Bayview Research Campus Alpha Center - 5210 Eastern Avenue - Baltimore, Maryland 21224 800.545.6569 - 410.550.2919 - 410.550.2924 (fax) NIH BPA#: 263-00054123 - www.paragonbioservices.com - pbi@paragonbioservices.com |
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© 2003 Paragon Bioservices, Inc. |
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