Bacteria & Yeast
Expression Systems

Search for Services

Starting from a gene sequence or cDNA, Paragon scientists will clone your gene (± affinity tags) into a commercially available expression vector or a custom vector that you provide.

Contact Paragon Bioservices to discuss your project
or to request a quote.

 

 
Scale:
From Fernbach Flasks to 15, 100, 250L Fermentors
 
Product:
-Cell Paste
-Cell Lysate in a suitable buffer
-Clarified culture supernatant
-Batch records & specification sheets

The veracity of constructs will be documented by restriction digest and/or DNA sequencing. Following transformation of expression cell lines (E. coli, S. cervisiae, P. Pastoris) and antibiotic selection of recombinants, the best protein expressing clones will be identified by SDS-PAGE and Western immunoblot.

The best clone will be further optimized for protein expression.

Paragon Home Page
 
Recombinant
Protein Expression
 
Bacteria & Yeast Expression 
 
Baculovirus -
Insect Cell
 
Mammalian
 
Transfection Services
  
Plasmid DNA
Production & Purification
 
 
 
 
 
 
 
 
 

Cell Culture & Fermentation Services
Recombinant Protein Expression | Protein Purification
Proteomics Services - Protein Characterization
Virus Production & Purification | Process Development
Functional Assays & Assay Development | Histology & Immunohistochemistry

site map | about us | contact us


Home Page - Paragon Bioservices  Paragon Bioservices, Inc - Johns Hopkins Bayview Research Campus
Alpha Center - 5210 Eastern Avenue - Baltimore, Maryland 21224
800.545.6569 - 410.550.2919 - 410.550.2924 (fax)
NIH BPA#: 263-00054123 - www.paragonbioservices.com - pbi@paragonbioservices.com

© 2003 Paragon Bioservices, Inc.