Baculovirus
Expression Systems

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Starting from a DNA sequence, cDNA or recombinant virus, Paragon scientists will clone your gene (± affinity tags) into a commercially available expression vector or a custom vector that you provide.

Contact Paragon Bioservices to discuss your project
or to request a quote.

 

 
Protein Production: 1L, 5L, 10L, 14L, 40L, 200L 
Product:
-Cell Paste
-Clarified cell culture supernatant
 
1. Starting from a DNA sequence, cDNA or recombinant virus
2. Cloning into commercially available expression vectors (± affinity tags). Veracity of construct demonstrated by restriction digest analysis and sequencing
3. Co-transfection with AcNPV DNA
4. Plaque purification of recombinant viruses
5. Expansion and PCR verification of recombinant viruses
6. Baculovirus expansion and titer - production of 1L high-titer stock
7. Time course and MOI study to optimize protein expression
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Recombinant
Protein Expression
 
Bacteria & Yeast Expression
 
Baculovirus -
Insect Cell
 
Mammalian
 
Transfection Services 
 
Plasmid DNA
Production & Purification
 
 
 
 
 
 
 
 
 

Cell Culture & Fermentation Services
Recombinant Protein Expression | Protein Purification
Proteomics Services - Protein Characterization
Virus Production & Purification | Process Development
Functional Assays & Assay Development | Histology & Immunohistochemistry

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Alpha Center - 5210 Eastern Avenue - Baltimore, Maryland 21224
800.545.6569 - 410.550.2919 - 410.550.2924 (fax)
NIH BPA#: 263-00054123 - www.paragonbioservices.com - pbi@paragonbioservices.com

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