Downstream Development

  • Column chromatography
    • Ion exchange
    • Hydrophobic Interaction (HIC)
    • Affinity
    • Size exclusion
    • Expanded bed absorption
    • Hydroxyapatite
  • Step or Linear gradient elution
  • TFF for concentration or buffer exchange
  • Impurity removal (viral, endotoxin, residual DNA, HCP)
  • Preliminary stability studies
  • Large-scale HPLC