Starting from a DNA sequence, cDNA or recombinant virus, Paragon scientists will clone your gene (± affinity tags) into a commercially available expression vector or a custom vector that you provide.
Cell lines
Scale
- 1L, 5L, 10L, 14L, 40L, 200L
Product
- Starting from a DNA sequence, cDNA or recombinant virus
- Cloning into commercially available expression vectors (± affinity tags). Veracity of construct demonstrated by restriction digest analysis and sequencing
- Co-transfection with AcNPV DNA
- Plaque purification of recombinant viruses
- Expansion and PCR verification of recombinant viruses
- Baculovirus expansion and titer - production of 1L high-titer stock
- Time course and MOI study to optimize protein expression