2-D Gel
Electrophoresis

Catalog #12607(W)
Catalog #12618(W)

Deliverables:
Digital Images of stained gels on disk
Immunoblot

• After performing 2-D electrophoresis as described above, the unstained resolved gel is transferred to PVDF membranes.
• Transferred proteins (spots) are then visualized by fluorescence staining, imaged and stored as tif files.
• The characterization of individual spots is then performed by Western Immunostaining of the same blot. A specific (primary) antibody is followed by a biotinylated secondary antibody and the complex is detected with Streptavidin HRP or ALK Phos using the appropriate substrates or Protein A Gold conjugates.
• Sequential probing with several primary antibodies (up to 3) can also be performed.
• Digital images of the blot are then superimposed on the fluorescent image previously captured to demonstrate the IEF and Molecular Weight location of a specific spot. Client would receive digital images of the resolved blot and immuno-detected spots.

Catalog #12507(Z3)
Catalog #12518(Z3)

Deliverables:
Annotated digital images of experimental vs. control experiments

• Following sample preparation, electrophoresis and staining of gels, unique spots will be identified and x/y annotated using Compugen’s Z3 software.