Protein Purification

Services for recombinant or natural proteins
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Combining its skills developed over the last 12 years in protein biochemistry, analytical chemistry, and functional assays, Paragon delivers efficient and cost effective solutions in protein purification and process development under Good Laboratory Practices in anticipation of future GMP manufacturing needs.
 
Provided by the client or produced at Paragon
  • cell pellets
  • bacterial paste
  • conditioned medium

Call 800-545-6569
or contact us
for further information
and quotation
Purification protocols
  • obtained from the client
  • obtained from the literature
  • developed de-novo at Paragon.

Before a project can proceed, the contracting laboratory must have approved all procedures to be implemented.

Final products are accompanied by batch records and specification sheets containing original documentation on yield, purity and identity.

In anticipation of your GMP manufacturing, Paragon provides
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Protein Purification
 
High Throughput
Protein Purification - IMAC 
 
 
 
 
 
 
 
 
 
 
Systems
Pharmacia FPLC System, Pharmacia Low-Pressure Chromatography System
Beckman HPLC System, BioSepra Trio System (process-scale FPLC)

Columns
Analytical-Scale, Preparative-Scale (5-, 9-, 18-, 25-, and 30-cm. diameter)

Special High-Performance, High-Dynamic Capacity Resins
Macro-Prep, Fastflow, Hyper D, Poros

Chemistry
Ion-Exchange (Q, S)
Affinity (Ni, Co, FLAG, maltose, glutathione, Protein A, Protein G)
Gel Filtration, Reverse-Phase, Non-Polar (C8, C18), HIC (Phenyl, Butyl)

Analytical to Preparative Scale
Microgram to gram quantities, Flow Rates: From 1 ml/min. to 3 liters/min.

High Throughput Protein Purification - IMAC

Analytical Techniques
 SDS-PAGE (Coomassie, Silver)
IEF, 2D Gel, Immunoblot, Protein Quantitation (A280, BCA, Bio-Rad)
ELISA

 

Cell Culture & Fermentation Services
Recombinant Protein Expression | Protein Purification
Proteomics Services - Protein Characterization
Virus Production & Purification | Process Development
Functional Assays & Assay Development | Histology & Immunohistochemistry
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Home Page - Paragon Bioservices  Paragon Bioservices, Inc - Johns Hopkins Bayview Research Campus
Alpha Center - 5210 Eastern Avenue - Baltimore, Maryland 21224
800.545.6569 - 410.550.2919 - 410.550.2924 (fax)
NIH BPA#: 263-00054123 - www.paragonbioservices.com - pbi@paragonbioservices.com

© 2003 Paragon Bioservices, Inc.